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We quantified total micro organism using universal bacterial primers [29] by qPCR in 114 adult fecal DNA samples which were analyzed for microbiota composition making use of Illumina MiSeq for 16S rRNA gene amplicon sequencing [fourteen]. The qPCR threshold cycle (Ct) values had been converted into the estimates of bacterial genomes existing in 1 g of feces as being a proxy of full bacterial counts.
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